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The characteristics of bone-marrow-derived macrophages from wild-type (WT) and cGAS-/- mice after 24 h activation by rabies virus (2 × 10 4 copies) with and without brain preparation from the WT control mice (6.25 mg), as indicated by the representative Western blot analysis and cell viability ( A ), supernatant proinflammatory cytokines (TNF-α, IL-6, and IL-10) ( B ), genes of pattern recognition receptors ( TLR3 , TLR-4 , TLR-7 , TLR-9 , and RIG-I ) ( C ), and genes of interferon (IFN) and cytokine production ( RIG-1 , MDA-5 , and NF-κB ) ( D ).

Journal: Biology

Article Title: Less Severe Inflammation in Cyclic GMP–AMP Synthase (cGAS)-Deficient Mice with Rabies, Impact of Mitochondrial Injury, and Gut–Brain Axis

doi: 10.3390/biology14111583

Figure Lengend Snippet: The characteristics of bone-marrow-derived macrophages from wild-type (WT) and cGAS-/- mice after 24 h activation by rabies virus (2 × 10 4 copies) with and without brain preparation from the WT control mice (6.25 mg), as indicated by the representative Western blot analysis and cell viability ( A ), supernatant proinflammatory cytokines (TNF-α, IL-6, and IL-10) ( B ), genes of pattern recognition receptors ( TLR3 , TLR-4 , TLR-7 , TLR-9 , and RIG-I ) ( C ), and genes of interferon (IFN) and cytokine production ( RIG-1 , MDA-5 , and NF-κB ) ( D ).

Article Snippet: To investigate the macrophage responses to rabies virus, BV2 (murine microglial cell line) (ATCC CRL-2467) and bone-marrow-derived macrophages (BMMs) were maintained in supplemented DMEM.

Techniques: Derivative Assay, Activation Assay, Virus, Control, Western Blot

The characteristics of bone-marrow-derived macrophages from wild-type (WT) and cGAS-/- mice after 24 h activation by rabies virus (2 × 10 4 copies) with and without brain preparation from the WT control mice (6.25 mg), as indicated by genes of M1 macrophage polarization ( IL-1β and iNOS ) and M2 macrophage polarization ( Arginase-1 , TGF-β , and Fizz ) ( A ), cGAS expression and supernatant 2’3’-cGAMP (a cGAS inducer) ( B ), markers of mitochondrial injury using MitoSox and expression of mitochondrial DNA (mtDNA) ( C ), and extracellular flux analysis using oxygen consumption rate (OCR) with several parameters ( D ).

Journal: Biology

Article Title: Less Severe Inflammation in Cyclic GMP–AMP Synthase (cGAS)-Deficient Mice with Rabies, Impact of Mitochondrial Injury, and Gut–Brain Axis

doi: 10.3390/biology14111583

Figure Lengend Snippet: The characteristics of bone-marrow-derived macrophages from wild-type (WT) and cGAS-/- mice after 24 h activation by rabies virus (2 × 10 4 copies) with and without brain preparation from the WT control mice (6.25 mg), as indicated by genes of M1 macrophage polarization ( IL-1β and iNOS ) and M2 macrophage polarization ( Arginase-1 , TGF-β , and Fizz ) ( A ), cGAS expression and supernatant 2’3’-cGAMP (a cGAS inducer) ( B ), markers of mitochondrial injury using MitoSox and expression of mitochondrial DNA (mtDNA) ( C ), and extracellular flux analysis using oxygen consumption rate (OCR) with several parameters ( D ).

Article Snippet: To investigate the macrophage responses to rabies virus, BV2 (murine microglial cell line) (ATCC CRL-2467) and bone-marrow-derived macrophages (BMMs) were maintained in supplemented DMEM.

Techniques: Derivative Assay, Activation Assay, Virus, Control, Expressing